Yongle Pan

pan Effects of Atmospheric Environments on the Properties of Single Bioaerosol Particles

About the Presenter:

Yongle Pan is a Research Physicist at Environmental Sensing Branch, CISD, US Army Research Laboratory since 2009. He was elected to be a Fellow of the US Army Research Laboratory in 2013. His PhD is in Laser Physics and Optics from East China Normal University in 1993. From 1993 to 1998 he was an Assistant Professor, then Associate Professor at Fudan University. From 1997 to 2008 he was a Visiting Professor, then Senior Research Scientist at Yale University. He has been working on inventing real-time, in-situ bio-aerosol detection and characterization systems for years, mainly based on single particle fluorescence spectra, two-dimensional angular optical scattering patterns, and Raman spectroscopy. Some of the advancements have been transitioned to DARPA, DTRA, DHS, USAMIID, ARL, ITT, and SNL. He has over 80 refereed journal papers, 3 book chapters, and 6 patents, over 1900 citations. He has successfully completed more than 20 research projects supported by DARPA, DTRA, JBTDS, DHS, ARO, AFOSR, DOE as a principal investigator (PI), Co-PI, or key personnel.

Full Presentation:

Dr. Pan declined to share his lecture online. Further information about his talk can be found by contacting the author.


The effects of the atmospheric environment (gases, sunlight, and humidity) on the properties (size, concentration, viability, and fluorescence intensity and spectra) of bioaerosols were studied using both laboratory and field systems. Each system consists of: an aerosol generator; a clean air generator (which removes trace gases such as volatile organics, ozone, and NOx,); one or two rotating reaction chamber(s) (rotating at 1 rpm to keep particles aloft for long, multi-hour, studies); a single-particle fluorescence spectrometer (which measures fluorescence spectra excited at 263 nm and 351 nm); a UV-APS (which measures size, concentration and total fluorescence exited at 355 nm); and all-glass impingers (AGI) aerosol collectors. The laboratory system is used to systematically study changes in fluorescence, size and viability of aerosols exposed to different controlled conditions (ozone concentrations, humidities and temperature). The field system is used to study property changes of bioaerosol exposed to solar flux, ozone, humidity, and temperature etc. of various actual atmospheric environments. Measurements of single bioaerosol particles such as Octapeptide, BtK, E.Coli, BG, MS2 using the laboratory and field systems will be presented. Results and possible future research will be discussed. This research was supported by the Basic and Supporting Science–program of the Defense Threat Reduction Agency (DTRA) and ARL mission funds.